Gel file name Lane # on gel Total # of lanes on gel Buffer Buffer Concentration Buffer Unit Salt Salt Concentration Salt Unit Detergent Detergent Concentration Detergent Unit Other Other Concentration Other Unit pH Mass Ladder Quantity Std Over-expressed Protein Systematic Name Tag Type Tag Location Antibody Other Capture Notes Single Reagent Flag 20110502_exampleGel_1 1 26 "250, 150, 100, 75, 50, 37, 25, 20, 15, 10" Yes 20110502_exampleGel_1 26 26 "BSA, 66.5, 100, ng" Yes 20110502_exampleGel_1 2 26 TRIS-Cl 40 mM Triton X-100 15 mM 8 no YOR098C SpA C-term Rabbit IgG Yes 20110502_exampleGel_1 4 26 TRIS-Cl 40 mM NaCl 300 mM Triton X-100 15 mM PEG 1K 2 % w/v 8 no NP_001272974.1 LAP (PMC2871289) C-term Llama polyclonal anti-GFP (PMC3605737) "Tandem purified with S-protein (EMD, 69704)" Yes 20110502_exampleGel_1 6 26 TRIS-Cl 40 mM Na2SO4 50 mM Triton X-100 15 mM 8 no NP_001137450.1 3xFLAG C-term "Mouse monoclonal anti-FLAG M2 (Sigma, F3165) " Yes 20110502_exampleGel_1 8 26 TRIS-Cl 40 mM Na2SO4 50 mM Triton X-100 15 mM 8 yes EAL23816.1 EGFP N-term anti-GFP nanobody (10.1074/mcp.M700342-MCP200) Yes